動(dòng)物視頻跟蹤和行為分析系統(tǒng)由高分辨率高幀頻工業(yè)相機(jī)、專業(yè)動(dòng)物行為采集及分析軟件,、紅外背光板等組成,,無(wú)需標(biāo)記即可對(duì)魚類、昆蟲(chóng),、哺乳動(dòng)物,、鳥(niǎo)類的行為進(jìn)行分析。使用時(shí)可直接使用軟件錄制高質(zhì)量的動(dòng)物活動(dòng)視頻并對(duì)視頻中的目標(biāo)動(dòng)物進(jìn)行識(shí)別跟蹤,,獲得目標(biāo)動(dòng)物的位置坐標(biāo)(X坐標(biāo)和Y坐標(biāo),;單個(gè)動(dòng)物可做3D跟蹤,即包括XYZ坐標(biāo))及隨時(shí)間變化的活動(dòng)軌跡,。系統(tǒng)可自動(dòng)計(jì)算個(gè)體,、處理組、感興趣區(qū)域相關(guān)的20余種行為參數(shù),,并可導(dǎo)出疊加分析數(shù)據(jù)的圖像和視頻以便于使用者進(jìn)行展示,。
功能特點(diǎn)
- 采用工業(yè)相機(jī),高分辨率,、高幀頻,、超高速傳輸
- 可跟蹤和分析魚類、昆蟲(chóng),、哺乳動(dòng)物,、鳥(niǎo)類及更多
- 基于對(duì)比度或者背景減除法發(fā)現(xiàn)目標(biāo),無(wú)需標(biāo)記物
- 可跟蹤任意大小和形狀的區(qū)域,,如迷宮,、水箱、呼吸室
- 對(duì)24孔板,、48孔板,、96孔板進(jìn)行高通量跟蹤和分析
- 3D跟蹤,可導(dǎo)出3D坐標(biāo)和路徑
- 可結(jié)合游泳儀,、植入式動(dòng)物體溫心率數(shù)據(jù)采集器等設(shè)備使用
系統(tǒng)配置
- 1彩色工業(yè)攝像機(jī)
- 專業(yè)級(jí)定焦鏡頭
- 專業(yè)動(dòng)物行為采集及分析軟件LoliTrack5
- 紅外背光板:用于透明多孔板和透明魚缸等的底部照明,,可消除反射,也可用于黑暗環(huán)境的視頻記錄
- 動(dòng)物活動(dòng)室/池(選配):定制化水箱/魚缸,、活動(dòng)室等
技術(shù)指標(biāo)
- 可自動(dòng)計(jì)算參數(shù)包括:
- 位置坐標(biāo)(包括前,、后、中線中心,、重心)
- 移動(dòng)距離
- 速度和速率(X方向,、Y方向)
- 加速度(X方向、Y方向)
- 移動(dòng)方向
- 身體取向
- 身體彎曲
- 轉(zhuǎn)彎速率
- 移動(dòng)方向和身體取向變化速率
- 活動(dòng)/非活動(dòng)時(shí)間和比例
- 感興趣區(qū)域花費(fèi)時(shí)間和占比
- 目標(biāo)動(dòng)物到感興趣區(qū)域的距離
- 個(gè)體間距離(IID),,包括平均,、眾數(shù)和最小
- 動(dòng)物行為觀測(cè)分析軟件具備多種基于反差法和背景減除法的過(guò)濾功能,。
- 軟件可跟蹤單個(gè)區(qū)域或多個(gè)區(qū)域內(nèi)的多個(gè)動(dòng)物,即使動(dòng)物具有相同(或不同)的形狀,、顏色和大小,。
- 可挑選單個(gè)個(gè)體用以跟蹤和分析,而非所有的個(gè)體,;可自定義跟蹤時(shí)長(zhǎng),,忽略或者只跟蹤某段時(shí)間;具備批量分析功能,。
- 軟件可識(shí)別特定身體的點(diǎn)位(如尾,、頭、中線中點(diǎn),、重心),,跟蹤這些點(diǎn)用以研究運(yùn)動(dòng)學(xué)(如擺尾頻率、身體擺動(dòng)等)或者特定的行為(如身體取向,、移動(dòng)方向等),。
- 軟件具備頻率(心率、擺尾頻率等)計(jì)算和容積(心博量等)計(jì)算功能,。
- 軟件具備錯(cuò)誤自查功能,,并提供手動(dòng)糾錯(cuò)的快捷方式。
- 軟件兼容大部分相機(jī)和視頻格式,,具備視頻記錄和編輯工具,,具備位置、速度,、加速度和轉(zhuǎn)彎速率的熱圖生成功能,并可輸出疊加了分析結(jié)果的視頻和圖片,,用戶可自定義Excel數(shù)據(jù)文件輸出內(nèi)容,。
- 軟件能夠?qū)蓚€(gè)相機(jī)或者兩個(gè)視頻文件的目標(biāo)動(dòng)物進(jìn)行3D視頻跟蹤和行為分析,導(dǎo)出3D坐標(biāo)和軌跡的3D模型,,在其他軟件做進(jìn)一步的可視化和分析,。
- 系統(tǒng)可對(duì)24、48或96孔板中的生物進(jìn)行跟蹤和分析,,對(duì)其活動(dòng)水平或者運(yùn)動(dòng)進(jìn)行快速篩選評(píng)估(常見(jiàn)于毒理學(xué)或者生物醫(yī)學(xué)檢測(cè)),。可輸入用戶自定義的處理描述以幫助分析數(shù)據(jù),。
- 1彩色高清高幀頻工業(yè)攝像機(jī):緊湊設(shè)計(jì),,分辨率2592 x 1944 (5.04 MP),幀率48FPS,,芯片尺寸1/2.5" (5.702 mm x 4.277 mm),,重量33g,。包括USB數(shù)據(jù)線、三腳架適配器,、壁掛式支架安裝,。
- 專業(yè)級(jí)定焦鏡頭:6mm、8mm,、12mm,、16mm、25mm,、50mm焦距可選,。
- 紅外背光板:大小16×16cm至56cm×56cm共6種尺寸可選,波長(zhǎng)850nm,。
應(yīng)用案例
高濃度人體可卡因代謝物苯甲酰愛(ài)康寧(BE)暴露使大型溞個(gè)體的活動(dòng)性顯著降低,、游泳速率顯著增大 (Environmental Pollution, 2018)
漸凍癥斑馬魚模型表現(xiàn)出病癥早期階段異常行為增多、晚期階段運(yùn)動(dòng)活性降低的表型(Glia, 2019)
產(chǎn)地:歐洲
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