動(dòng)物視頻跟蹤和行為分析系統(tǒng)由高分辨率高幀頻工業(yè)相機(jī),、專業(yè)動(dòng)物行為采集及分析軟件,、紅外背光板等組成,，無需標(biāo)記即可對魚類,、昆蟲,、哺乳動(dòng)物,、鳥類的行為進(jìn)行分析,。使用時(shí)可直接使用軟件錄制高質(zhì)量的動(dòng)物活動(dòng)視頻并對視頻中的目標(biāo)動(dòng)物進(jìn)行識別跟蹤,，獲得目標(biāo)動(dòng)物的位置坐標(biāo)（X坐標(biāo)和Y坐標(biāo),；單個(gè)動(dòng)物可做3D跟蹤，即包括XYZ坐標(biāo)）及隨時(shí)間變化的活動(dòng)軌跡,。系統(tǒng)可自動(dòng)計(jì)算個(gè)體,、處理組、感興趣區(qū)域相關(guān)的20余種行為參數(shù),，并可導(dǎo)出疊加分析數(shù)據(jù)的圖像和視頻以便于使用者進(jìn)行展示,。
功能特點(diǎn)
- 采用工業(yè)相機(jī)，高分辨率,、高幀頻,、超高速傳輸
- 可跟蹤和分析魚類、昆蟲,、哺乳動(dòng)物,、鳥類及更多
- 基于對比度或者背景減除法發(fā)現(xiàn)目標(biāo),，無需標(biāo)記物
- 可跟蹤任意大小和形狀的區(qū)域，如迷宮,、水箱,、呼吸室
- 對24孔板、48孔板,、96孔板進(jìn)行高通量跟蹤和分析
- 3D跟蹤,，可導(dǎo)出3D坐標(biāo)和路徑
- 可結(jié)合游泳儀、植入式動(dòng)物體溫心率數(shù)據(jù)采集器等設(shè)備使用
系統(tǒng)配置
- 1彩色工業(yè)攝像機(jī)
- 專業(yè)級定焦鏡頭
- 專業(yè)動(dòng)物行為采集及分析軟件LoliTrack5
- 紅外背光板：用于透明多孔板和透明魚缸等的底部照明,，可消除反射,，也可用于黑暗環(huán)境的視頻記錄
- 動(dòng)物活動(dòng)室/池（選配）：定制化水箱/魚缸、活動(dòng)室等
技術(shù)指標(biāo)
- 可自動(dòng)計(jì)算參數(shù)包括：
- 位置坐標(biāo)（包括前,、后,、中線中心、重心）
- 移動(dòng)距離
- 速度和速率（X方向,、Y方向）
- 加速度（X方向,、Y方向）
- 移動(dòng)方向
- 身體取向
- 身體彎曲
- 轉(zhuǎn)彎速率
- 移動(dòng)方向和身體取向變化速率
- 活動(dòng)/非活動(dòng)時(shí)間和比例
- 感興趣區(qū)域花費(fèi)時(shí)間和占比
- 目標(biāo)動(dòng)物到感興趣區(qū)域的距離
- 個(gè)體間距離（IID），包括平均,、眾數(shù)和最小
- 動(dòng)物行為觀測分析軟件具備多種基于反差法和背景減除法的過濾功能,。
- 軟件可跟蹤單個(gè)區(qū)域或多個(gè)區(qū)域內(nèi)的多個(gè)動(dòng)物，即使動(dòng)物具有相同（或不同）的形狀,、顏色和大小,。
- 可挑選單個(gè)個(gè)體用以跟蹤和分析，而非所有的個(gè)體,；可自定義跟蹤時(shí)長,，忽略或者只跟蹤某段時(shí)間；具備批量分析功能,。
- 軟件可識別特定身體的點(diǎn)位（如尾,、頭、中線中點(diǎn),、重心）,，跟蹤這些點(diǎn)用以研究運(yùn)動(dòng)學(xué)（如擺尾頻率、身體擺動(dòng)等）或者特定的行為（如身體取向,、移動(dòng)方向等）,。
- 軟件具備頻率（心率、擺尾頻率等）計(jì)算和容積（心博量等）計(jì)算功能,。
- 軟件具備錯(cuò)誤自查功能,，并提供手動(dòng)糾錯(cuò)的快捷方式。
- 軟件兼容大部分相機(jī)和視頻格式,，具備視頻記錄和編輯工具,，具備位置,、速度、加速度和轉(zhuǎn)彎速率的熱圖生成功能,，并可輸出疊加了分析結(jié)果的視頻和圖片，用戶可自定義Excel數(shù)據(jù)文件輸出內(nèi)容,。
- 軟件能夠?qū)蓚€(gè)相機(jī)或者兩個(gè)視頻文件的目標(biāo)動(dòng)物進(jìn)行3D視頻跟蹤和行為分析,，導(dǎo)出3D坐標(biāo)和軌跡的3D模型，在其他軟件做進(jìn)一步的可視化和分析,。
- 系統(tǒng)可對24,、48或96孔板中的生物進(jìn)行跟蹤和分析，對其活動(dòng)水平或者運(yùn)動(dòng)進(jìn)行快速篩選評估（常見于毒理學(xué)或者生物醫(yī)學(xué)檢測）,?？奢斎胗脩糇远x的處理描述以幫助分析數(shù)據(jù)。
- 1彩色高清高幀頻工業(yè)攝像機(jī)：緊湊設(shè)計(jì),，分辨率2592 x 1944 (5.04 MP),，幀率48FPS，芯片尺寸1/2.5" (5.702 mm x 4.277 mm),，重量33g,。包括USB數(shù)據(jù)線、三腳架適配器,、壁掛式支架安裝,。
- 專業(yè)級定焦鏡頭：6mm、8mm,、12mm,、16mm、25mm,、50mm焦距可選,。
- 紅外背光板：大小16×16cm至56cm×56cm共6種尺寸可選，波長850nm,。
應(yīng)用案例
高濃度人體可卡因代謝物苯甲酰愛康寧（BE）暴露使大型溞個(gè)體的活動(dòng)性顯著降低,、游泳速率顯著增大 (Environmental Pollution, 2018)
漸凍癥斑馬魚模型表現(xiàn)出病癥早期階段異常行為增多、晚期階段運(yùn)動(dòng)活性降低的表型(Glia, 2019)
產(chǎn)地：歐洲
參考文獻(xiàn)
- Bucking, C., Wood, C.M., and Grosell, M. (2012). Diet influences salinity preference of an estuarine fish, the killifish Fundulus heteroclitus. Journal of Experimental Biology 215, 1965–1974.
- Di Cicco, M., Di Lorenzo, T., Fiasca, B., Ruggieri, F., Cimini, A., Panella, G., Benedetti, E., and Galassi, D.M.P. (2021). Effects of diclofenac on the swimming behavior and antioxidant enzyme activities of the freshwater interstitial crustacean Bryocamptus pygmaeus (Crustacea, Harpacticoida). Science of The Total Environment 799, 149461.
- Dipp, V.R., Valles, S., Ortiz-Kerbertt, H., Suarez, J.V., and Bardullas, U. (2018). Neurobehavioral Alterations in Zebrafish Due to Long-Term Exposure to Low Doses of Inorganic Arsenic. Zebrafish 15, 575–585.
- Hansen, L.R., and Roslev, P. (2016). Behavioral responses of juvenile Daphnia magna after exposure to glyphosate and glyphosate-copper complexes. Aquatic Toxicology 179, 36–43.
- Herbert, N.A., Skj?raasen, J.E., Nilsen, T., Salvanes, A.G.V., and Steffensen, J.F. (2011). The hypoxia avoidance behaviour of juvenile Atlantic cod (Gadus morhua L.) depends on the provision and pressure level of an O2 refuge. Mar Biol 158, 737–746.
- Huang, Y., Cartlidge, R., Walpitagama, M., Kaslin, J., Campana, O., and Wlodkowic, D. (2018). Unsuitable use of DMSO for assessing behavioral endpoints in aquatic model species. Science of The Total Environment 615, 107–114.
- Khor, Y.M., Soga, T., and Parhar, I.S. (2013). Caffeine neuroprotects against dexamethasone-induced anxiety-like behaviour in the Zebrafish (Danio rerio). General and Comparative Endocrinology 181, 310–315.
- Kim, S., Chung, A., Na, J.E., Lee, S.J., Jeong, S.H., Kim, E., Sun, W., Rhyu, I.J., and Park, H. (2019). Myelin degeneration induced by mutant superoxide dismutase 1 accumulation promotes amyotrophic lateral sclerosis. Glia. 23669.
- Kruusmaa, M., Gkliva, R., Tuhtan, J.A., Tuvikene, A., and Alfredsen, J.A. Salmon behavioural response to robots in an aquaculture sea cage. Royal Society Open Science 7, 191220.
- Millot, S., Cerqueira, M., Castanheira, M.-F., ?verli, ?., Oliveira, R.F., and Martins, C.I.M. (2014). Behavioural Stress Responses Predict Environmental Perception in European Sea Bass (Dicentrarchus labrax). PLOS ONE 9, e108800.
- Nam, T.-S., Zhang, J., Chandrasekaran, G., Jeong, I.Y., Li, W., Lee, S.-H., Kang, K.-W., Maeng, J.-S., Kang, H., Shin, H.-Y., et al. (2020). A zebrafish model of nondystrophic myotonia with sodium channelopathy. Neuroscience Letters 714, 134579.
- Nielsen, M.E., and Roslev, P. (2018). Behavioral responses and starvation survival of Daphnia magna exposed to fluoxetine and propranolol. Chemosphere 211, 978–985.
- Parolini, M., De Felice, B., Ferrario, C., Salgueiro-González, N., Castiglioni, S., Finizio, A., and Tremolada, P. (2018). Benzoylecgonine exposure induced oxidative stress and altered swimming behavior and reproduction in Daphnia magna. Environmental Pollution 232, 236–244.
- Qian, L., Liu, J., Lin, Z., Chen, X., Yuan, L., Shen, G., Yang, W., Wang, D., Huang, Y., Pang, S., et al. (2020). Evaluation of the spinal effects of phthalates in a zebrafish embryo assay. Chemosphere 249, 126144.
- Qian, L., Qi, S., Wang, Z., Magnuson, J.T., Volz, D.C., Schlenk, D., Jiang, J., and Wang, C. (2021). Environmentally relevant concentrations of boscalid exposure affects the neurobehavioral response of zebrafish by disrupting visual and nervous systems. Journal of Hazardous Materials 404, 124083.
- Rountos, K.J., Kim, J.J., Hattenrath-Lehmann, T.K., and Gobler, C.J. (2019). Effects of the harmful algae, Alexandrium catenella and Dinophysis acuminata, on the survival, growth, and swimming activity of early life stages of forage fish. Marine Environmental Research 148, 46–56.
- Seebacher, F., Kazerouni, E.G., and Franklin, C.E. (2016). Ultraviolet B radiation alters movement and thermal selection of zebrafish (Danio rerio). Biology Letters 12, 20160258.
- Sotto, R.B.D., Medriano, C.D., Cho, Y., Kim, H., Chung, I.-Y., Seok, K.-S., Song, K.G., Hong, S.W., Park, Y., and Kim, S. (2017). Sub-lethal pharmaceutical hazard tracking in adult zebrafish using untargeted LC–MS environmental metabolomics. Journal of Hazardous Materials 339, 63–72.
- Yang, F., Zeng, Y., Huai, W., Zha, W., and Wan, Y. (2022). Responses of cyprinid (Ancherythroculter nigrocauda) to flow with a semi-circular cylinder patch. Journal of Fish Biology 100, 884–893.
- 郝文超, 王從鋒, 劉慧杰, 王瑤, and 馮三杰 (2018). 不同運(yùn)動(dòng)模式下鰱幼魚游泳特性研究. 淡水漁業(yè) 48, 3–9. .
