時間:2018-11-14
作者:易科泰
點擊量:
簡介:
前言
LCi-T 便攜式光合儀是最小巧,、輕便的便攜式光合作用測定儀,,用以測量植物葉片的光合速率,、蒸騰速率,、氣孔導(dǎo)度等與植物光合作用相關(guān)的參數(shù),。儀器應(yīng)用IRGA(紅外氣體分析)原理,,精密測量葉片表面CO2濃度及水分的變化情況來考察葉片與植物光合作用相關(guān)的參數(shù),。特殊的設(shè)計可在高濕度、高塵埃環(huán)境使用,。既可在研究中使用,,又是很好的教學(xué)儀器。
上圖左為全套光合儀主機配件及便攜箱等,,上圖中為光合儀主機和手柄,,上圖右為操作人員進行野外實驗
應(yīng)用領(lǐng)域
l 植物光合生理研究
l 植物抗脅迫研究
l 碳源碳匯研究
l 植物對全球氣候變化的相應(yīng)及其機理
l 作物新品種篩選
l 配備手持式葉綠素熒光儀,內(nèi)置了所有通用葉綠素熒光分析實驗程序,,包括兩套熒光淬滅分析程序,、3套光響應(yīng)曲線程序、OJIP-test等
l 彩色觸摸屏,,根據(jù)環(huán)境光線自動調(diào)整亮度,,既方便野外查看數(shù)據(jù),又延長續(xù)航時間
l 任選RGB(Red Green Blue)或白色光源之一作為標配
l 便攜式設(shè)計,,體積輕巧,,僅重2.4 Kg
l 微型IRGA置于測量手柄中,大大縮短CO2測量的反應(yīng)時間
l 可在惡劣環(huán)境下使用
l 可方便互換不同種類的葉室
l 葉室材料經(jīng)精心選擇,,以確保CO2及水分的測量精度
l 數(shù)據(jù)存儲量大,,采用即插即拔SD卡
l 操作簡單,,維護方便,葉室所有區(qū)域都很容易清潔
l 采用低能耗技術(shù),,野外單電池持續(xù)工作時間可達10小時
l 內(nèi)置GPS
上圖為英國劍橋大學(xué)植物科學(xué)系M. Davey博士在南極洲對藻類光合作用研究時的工作圖片,,因LC系列光合儀輕便小巧,堅固耐用,,續(xù)航持久等特點被列為首選,。
l 測量參數(shù):光合速率、蒸騰速率,、胞間CO2濃度,、氣孔導(dǎo)度、葉片溫度,、葉室溫度,、光合有效輻射、氣壓,、光響應(yīng)曲線等
l 手持葉綠素熒光儀(選配)
1. 測量參數(shù)包括F0,、Ft、Fm,、Fm’,、QY_Ln、QY_Dn,、NPQ、Qp,、Rfd,、RAR、Area,、M0,、Sm、PI,、ABS/RC等50多個葉綠素熒光參數(shù),,及3種給光程序的光響應(yīng)曲線、2種熒光淬滅曲線,、OJIP曲線等
2. 高時間分辨率,,可達10萬次每秒,自動繪出OJIP曲線并給出26個OJIP-test測量參數(shù)包括F0,、Fj,、Fi、Fm,、Fv,、Vj,、Vi、Fm/F0,、Fv/F0,、Fv/Fm、M0,、Area,、Fix Area、Sm,、Ss,、N、Phi_P0,、Psi_0,、Phi_E0、Phi-D0,、Phi_Pav,、PI_Abs、ABS/RC,、TR0/RC,、ET0/RC、DI0/RC等
l CO2測量范圍:0-2000ppm
l CO2測量分辨率:1ppm
l CO2采用紅外分析系統(tǒng),,差分開路測量系統(tǒng),,自動置零,自動氣壓和溫度補償
lH2O測量范圍:0-75 mbar
l H2O測量分辨率:0.1mbar
l H2O測量采用雙激光調(diào)諧快速響應(yīng)水蒸氣傳感器
l PAR測量范圍:0-3000 μmol m-2 s-1
l 葉室溫度:-5 - 50℃ 精度:±0.2℃
l 葉片溫度:-5 - 50℃
l 葉室中空氣流量:68 – 340 ml / min
l 空氣流量精度:全量程的±2%
l 預(yù)熱時間:20℃時5分鐘
l 數(shù)據(jù)存儲:SD卡,,最大支持32GB擴展,,可存儲16,000,000組典型數(shù)據(jù)
l 數(shù)據(jù)接口:mini-USB接口,RS232標準接口
l 圖形顯示:彩色WQVGA LCD觸摸屏,,480 x 272像素,,尺寸95 x 53.9 mm,對角線長109mm,,可實時圖形顯示各測量參數(shù)
l 可選配便攜式光源:具有PLU控制單元,,控光范圍0-2400 μmol m-2 s-1
l 可選配葉室
1. 寬葉葉室:長×寬為2.5×2.5cm,適用于闊葉及大多數(shù)葉片類型
2. 窄葉葉室:長×寬為5.8×1cm,,適用寬度小于1cm的條形葉
3. 針葉葉室:長約69mm,,直徑47mm,適用于簇狀針葉(白光光源)
4. 小型葉葉室:葉室直徑為16.5mm,,測量面積2.16cm2
5. 土壤呼吸/小型植物室:測量測量土壤呼吸,,或者高度低于55mm的整株草本植物光合作用,底面直徑為11cm
6. 多功能測量室:長×寬×高為15×15×7cm,,分為上下兩部分,,上部測量小型植物光合作用,,下部分測量土壤呼吸
7. 果實測量室:上下兩部分組成,上部透明,,下部為金屬,,可測量果實最大直徑為11cm,最大高度為11.5cm
8. 冠層測量室:底面直徑12.7cm,,高12.2cm,,適用于地表冠層
9. 熒光儀聯(lián)用適配器:適用于連接多種葉綠素熒光儀
上圖從左到右依次為寬葉室、窄葉室,、LED光源,、熒光儀聯(lián)用葉室、小型葉室
上圖從左到右依次為針葉室,、果實測量室,、土壤呼吸室、多功能測量室,、冠層室
l 供電系統(tǒng):內(nèi)置12V 2.8AH鉛酸電池,,可持續(xù)工作10小時左右
l 操作環(huán)境:5到45℃
l 主機尺寸:240×125×140mm,2.4Kg
l 主機顯示參數(shù):環(huán)境CO2和水蒸汽,;CO2和水蒸汽變化,;葉室和葉片的溫度;氣流速率,;大氣壓,;光合有效輻射;光合速率,;胞間CO2濃度,;蒸騰速率;氣孔導(dǎo)度,;電池狀態(tài)等
Leaf life span optimizes annual biomass production rather than plant photosynthetic capacity in an evergreen shrub, Marty C. et al. 2010, New Phytologist, 187(2): 407-416
本文研究了Rhododendron ferrugineum(高山玫瑰杜鵑,杜鵑屬模式種)凈光合能力與葉片壽命的關(guān)系,,發(fā)現(xiàn)有更多較老葉片的種群其光合能力更強(圖中深色區(qū)域為一年葉片和二年葉片),。
英國
1) 與葉綠素熒光儀組成光合作用與葉綠素熒光測量系統(tǒng)
2) 與FluorCam聯(lián)用組成光合作用與葉綠素熒光成像測量系統(tǒng)
3) 可選配高光譜成像實現(xiàn)從單葉片到復(fù)合冠層的光合作用時空變化研究
4) 可選配O2測量單元
5) 可選配紅外熱成像單元以分析氣孔導(dǎo)度動態(tài)
6) 可選配PSI智能LED光源
7) 可選配FluorPen、SpectraPen,、PlantPen等手持式植物(葉片)測量儀器,,全面分析植物葉片生理生態(tài)
8) 可選配ECODRONE?無人機平臺搭載高光譜和紅外熱成像傳感器進行時空格局調(diào)查研究
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